Virus Details


VHFID8722

Host Factor Information

Gene Name CSNK2A1
HF Protein Name Casein kinase II subunit alpha
HF Function Essential for virus infection
Uniprot ID P68400
Protein Sequence View Fasta Sequence
NCBI Gene ID 1457
Host Factor (HF) Name in Paper CSNK2A1
Gene synonyms CK2A1
Ensemble Gene ID ENSG00000101266
Ensemble Transcript ENST00000217244 [P68400-1];ENST00000349736 [P68400-1];ENST00000400217 [P68400-2]
KEGG ID Go to KEGG Database
Gene Ontology ID(s) GO:0004674, GO:0005524, GO:0005634, GO:0005654, GO:0005829, GO:0005886, GO:0005956, GO:0006351, GO:0006355, GO:0006457, GO:0006468, GO:0006656, GO:0006915, GO:0007049, GO:0007165, GO:0008284, GO:0016055, GO:0016236, GO:0016301, GO:0018107, GO:0030177, GO:0030307, GO:0042802, GO:0043154, GO:0045732, GO:0047485, GO:0048511, GO:0051879, GO:0061077, GO:1901796, GO:1905818,
MINT ID P68400
STRING Click to see interaction map
GWAS Analysis Click to see gwas analysis
OMIM ID 115440
PANTHER ID N.A.
PDB ID(s) 1JWH, 1NA7, 1PJK, 2PVR, 2ZJW, 3AMY, 3AT2, 3AT3, 3AT4, 3AXW, 3BQC, 3C13, 3FWQ, 3H30, 3JUH, 3MB6, 3MB7, 3NGA, 3NSZ, 3OWJ, 3OWK, 3OWL, 3PE1, 3PE2, 3PE4, 3Q04, 3Q9W, 3Q9X, 3Q9Y, 3Q9Z, 3QA0, 3R0T, 3RPS, 3TAX, 3U4U, 3U87, 3U9C, 3W8L, 3WAR, 3WIK, 3WIL, 3WOW, 4DGL, 4FBX, 4GRB, 4GUB, 4GYW, 4GYY, 4GZ3, 4IB5, 4KWP, 4MD7, 4MD8, 4MD9, 4NH1, 4RLL, 4UB7, 4UBA, 5B0X, 5CLP, 5CQU, 5CQW, 5CS6, 5CSH, 5CSP, 5CSV, 5CT0, 5CTP, 5CU0, 5CU2, 5CU3, 5CU4, 5CU6, 5CVF, 5CVG, 5CVH, 5CX9, 5H8B, 5H8E, 5H8G, 5HGV, 5KU8, 5KWH, 5M44, 5M4C, 5M4F, 5M4I, 5MMF, 5MMR, 5MO5, 5MO6, 5MO7, 5MO8, 5MOD, 5MOE, 5MOH, 5MOT, 5MOV, 5MOW, 5MP8, 5MPJ, 5N1V, 5N9K, 5N9L, 5N9N, 5NQC, 5OMY, 5ONI, 5OQU, 5ORH, 5ORJ, 5ORK, 5OS7, 5OS8, 5OSL, 5OSZ, 5OT5, 5OT6, 5OTD, 5OTH, 5OTI, 5OTL, 5OTO, 5OTQ, 5OTR, 5OTY, 5OTZ, 5OUE, 5OUL, 5OUM, 5OUU, 5OYF, 5T1H, 5VIE, 5VIF, 6EHK, 6EHU, 6EII,
pfam ID PF00069,
Drug Bank ID DB03127, DB08846, DB04395, DB02709, DB04462,
ChEMBL ID CHEMBL3629
Organism Homo sapiens (Human)

Pathogen Information

Virus Name West Nile virus
Virus Short Name WNV
Order Unassigned
Virus Family Flaviviridae
Virus Subfamily N.A.
Genus Flavivirus
Species West Nile virus
Host Human, mammals, mosquitoes and ticks
Cell Tropism Epithelial cells in the skin, kidney, intestine, and testes
Associated Disease Fever, headache, vomiting, rash
Mode of Transmission Arthropod bite, mainly mosquitoes
VIPR DB link http://www.viprbrc.org/brc/home.spg?decorator=flavi
ICTV DB link https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/flaviviridae
Virus Host DB link http://www.genome.jp/virushostdb/view/?virus_lineage=Flaviviridae

Publication Information

Paper Title RNA interference screen for human genes associated with West Nile virus infection
Author's Name Manoj N Krishnan, Aylwin Ng, Bindu Sukumaran, Felicia D Gilfoy, Pradeep D Uchil, Hameeda Sultana, Abraham L Brass, Rachel Adametz, Melody Tsui, Feng Qian, Ruth R Montgomery, Sima Lev, Peter W Mason,Raymond A Koski, Stephen J Elledge, Ramnik J Xavier, Herve Agaisse and Erol Fikrig
Journal Name NATURE
Pubmed ID 18690214
Abstract West Nile virus (WNV), and related flaviviruses such as tick-borne encephalitis, Japanese encephalitis, yellow fever and dengue viruses, constitute a significant global human health problem. However, our understanding of the molecular interaction of such flaviviruses with mammalian host cells is limited. WNV encodes only 10 proteins, implying that it may use many cellular proteins for infection. WNV enters the cytoplasm through pH-dependent endocytosis, undergoes cycles of translation and replication, assembles progeny virions in association with endoplasmic reticulum, and exits along the secretory pathway. RNA interference (RNAi) presents a powerful forward genetics approach to dissect virus-host cell interactions. Here we report the identification of 305 host proteins that affect WNV infection, using a human-genome-wide RNAi screen. Functional clustering of the genes revealed a complex dependence of this virus on host cell physiology, requiring a wide variety of molecules and cellular pathways for successful infection. We further demonstrate a requirement for the ubiquitin ligase CBLL1 in WNV internalization, a post-entry role for the endoplasmic-reticulum-associated degradation pathway in viral infection, and the monocarboxylic acid transporter MCT4 as a viral replication resistance factor. By extending this study to dengue virus, we show that flaviviruses have both overlapping and unique interaction strategies with host cells. This study provides a comprehensive molecular portrait of WNV-human cell interactions that forms a model for understanding single plus-stranded RNA virus infection, and reveals potential antiviral targets.
Used Model HeLa cells
DOI 10.1038/nature07207