| Gene Name | UBE4A |
| HF Protein Name | Ubiquitin conjugation factor E4 A |
| HF Function | Inhibits viral infection |
| Uniprot ID | Q14139 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 9354 |
| Host Factor (HF) Name in Paper | UBE4A |
| Gene synonyms | KIAA0126 |
| Ensemble Gene ID | ENSG00000110344 |
| Ensemble Transcript | ENST00000252108 [Q14139-1];ENST00000431736 [Q14139-2] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000151, GO:0000209, GO:0005634, GO:0005737, GO:0006511, GO:0030433, GO:0034450, |
| MINT ID | Q14139 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 603753 |
| PANTHER ID | N.A. |
| PDB ID(s) | 1WGM, |
| pfam ID | PF04564, PF10408, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Adeno-associated virus 2 |
| Virus Short Name | AAV2 |
| Order | Unassigned |
| Virus Family | Parvoviridae |
| Virus Subfamily | Parvovirinae |
| Genus | Dependovirus |
| Species | Adeno-associated virus 2 |
| Host | Human, vertebrates |
| Cell Tropism | N.A. |
| Associated Disease | Asymptomatic |
| Mode of Transmission | Respiratory, oral droplets |
| VIPR DB link | N.A. |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/ssdna-viruses-2011/w/ssdna_viruses/151/parvoviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Parvoviridae |
| Paper Title | Genome wide RNAi screening identifies host restriction factors critical for in vivo AAV transduction |
| Author's Name | Miguel Manoa, Rudy Ippodrinoa , Lorena Zentilina , Serena Zacchigna, Mauro Giacca |
| Journal Name | PNAS |
| Pubmed ID | 26305933 |
| Abstract | Viral vectors based on the adeno-associated virus (AAV) hold great promise for in vivo gene transfer several unknowns, however, still limit the vectors broader and more efficient application. Here, we report the results of a high-throughput, whole-genome siRNA screening aimed at identifying cellular factors regulating AAV transduction. We identified 1,483 genes affecting vector efficiency more than 4-fold and up to 50-fold, either negatively or positively. Most of these factors have not previously been associated to AAV infection. The most effective siRNAs were independent from the virus serotype or analyzed cell type and were equally evident for single-stranded and self-complementary AAV vectors. A common characteristic of the most effective siRNAs was the induction of cellular DNA damage and activation of a cell cycle checkpoint. This information can be exploited for the development of more efficient AAV-based gene delivery procedures. Administration of the most effective siRNAs identified by the screening to the liver significantly improved in vivo AAV transduction efficiency. |
| Used Model | HeLa, U2OS and MRC5 cell lines |
| DOI | 10.1073/pnas.1503607112 |
