| Gene Name | RPLP2 |
| HF Protein Name | 60S acidic ribosomal protein P2 |
| HF Function | Essential for viral entry |
| Uniprot ID | P05387 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 6181 |
| Host Factor (HF) Name in Paper | RPLP2 |
| Gene synonyms | D11S2243E RPP2 |
| Ensemble Gene ID | ENSG00000177600 |
| Ensemble Transcript | ENST00000321153;ENST00000530797 |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000184, GO:0002181, GO:0003735, GO:0005829, GO:0005925, GO:0006364, GO:0006412, GO:0006413, GO:0006414, GO:0006614, GO:0016020, GO:0019083, GO:0022625, GO:0070062, |
| MINT ID | P05387 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 180530 |
| PANTHER ID | N.A. |
| PDB ID(s) | 1S4J, 2JDL, 2LBF, 2W1O, 4BEH, 4V6X, 5DDZ, 5GU4, |
| pfam ID | N.A., |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Yellow fever virus |
| Virus Short Name | YFV |
| Order | Unassigned |
| Virus Family | Flaviviridae |
| Virus Subfamily | N.A. |
| Genus | Flavivirus |
| Species | Yellow fever virus |
| Host | Human, mammals, mosquitoes and ticks |
| Cell Tropism | N.A. |
| Associated Disease | Hemorrhagic fever, encephalitis |
| Mode of Transmission | Arthropod bite, mainly mosquitoes |
| VIPR DB link | http://www.viprbrc.org/brc/home.spg?decorator=flavi |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_online_report/positive-sense-rna-viruses/w/flaviviridae |
| Virus Host DB link | http://www.genome.jp/virushostdb/view/?virus_lineage=Flaviviridae |
| Paper Title | RPLP1 and RPLP2 are essential flavivirus host factors that promote early viral protein accumulation |
| Author's Name | Rafael K. Campos, Benjamin Wong, Xuping Xie, Yi-Fan Lu, Pei-Yong Shi, Julien Pompon, Mariano A. Garcia-Blanco, Shelton S. Bradrick |
| Journal Name | Journal Of Virology |
| Pubmed ID | 27974556 |
| Abstract | The Flavivirus genus contains several arthropod-borne viruses that pose global health threats, including dengue viruses (DENV), yellow fever virus (YFV), and Zika virus (ZIKV). In order to understand how these viruses replicate in human cells, we previously conducted genome-scale RNA interference screens to identify candidate host factors. In these screens, we identified ribosomal proteins RPLP1and RPLP2 (RPLP1/2) to be among the most crucial putative host factors required for DENV and YFV infection. RPLP1/2 are phosphoproteins that bind the ribosome through interaction with another ribosomal protein, RPLP0, to form a structure termed the ribosomal stalk. RPLP1/2 were validated as essential host factors for DENV, YFV, and ZIKV infection in two human cell lines: A549 lung adenocarcinoma and HuH-7 hepatoma cells, and for productive DENV infection of Aedes aegypti mosquitoes. Depletion of RPLP1/2 caused moderate cell-line-specific effects on global protein synthesis, as determined by metabolic labeling. In A549 cells, global translation was increased, while in HuH-7 cells it was reduced, albeit both of these effects were modest. In contrast, RPLP1/2 knockdown strongly reduced early DENV protein accumulation, suggesting a requirement for RPLP1/2 in viral translation. Furthermore, knockdown of RPLP1/2 reduced levels of DENV structural proteins expressed from an exogenous transgene. We postulate that these ribosomal proteins are required for efficient translation elongation through the viral open reading frame. In summary, this work identifies RPLP1/2 as critical flaviviral host factors required for translation. |
| Used Model | Vero, A549, HuH-7, and HuH-7.5 cells |
| DOI | 10.1128/JVI.01706-16 |
