| Gene Name | DCD |
| HF Protein Name | Dermcidin |
| HF Function | Inhibits virus replication |
| Uniprot ID | P81605 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 117159 |
| Host Factor (HF) Name in Paper | DCD |
| Gene synonyms | AIDD DSEP |
| Ensemble Gene ID | ENSG00000161634 |
| Ensemble Transcript | ENST00000293371 [P81605-1];ENST00000456047 [P81605-2];ENST00000546807 [P81605-3] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0003723, GO:0005576, GO:0005615, GO:0008233, GO:0019730, GO:0031640, GO:0042742, GO:0050829, GO:0050830, GO:0050832, GO:0061844, GO:0070062, |
| MINT ID | N.A. |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 606634 |
| PANTHER ID | PTHR40711:SF1 |
| PDB ID(s) | 2KSG, 2NDK, 2YMK, |
| pfam ID | PF15291, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Zaire ebolavirus |
| Virus Short Name | ZEBOV |
| Order | Bunyavirales |
| Virus Family | Filoviridae |
| Virus Subfamily | N.A. |
| Genus | Ebolavirus |
| Species | Zaire ebolavirus |
| Host | Bats, human and primates |
| Cell Tropism | N.A. |
| Associated Disease | Hemorragic fever |
| Mode of Transmission | Zoonosis, contact with body fluids |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=filo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/197/filoviridae |
| Virus Host DB link | N.A. |
| Paper Title | Identification of essential filovirion associated host factors by serial proteomic analysis and RNAi screen |
| Author's Name | Kevin B. Spurgers, Tim Alefantis, Brian D. Peyser, Gordon T. Ruthel, Alison A. Bergeron, Julie A. Costantino, Sven Enterlein, Krishna P. Kota, R. C. Dutch Boltz, M. Javad Aman, Vito G. DelVecchio and Sina Bavari |
| Journal Name | Molecular and Cellular Proteomics |
| Pubmed ID | 20702783 |
| Abstract | An assessment of the total protein composition of filovirus (ebolavirus and marburgvirus) virions is currently lacking. In this study, liquid chromatography-linked tandem mass spectrometry of purified ebola and marburg virions was performed to identify associated cellular proteins. Host proteins involved in cell adhesion, cytoskeleton, cell signaling, intracellular trafficking, membrane organization, and chaperones were identified. Significant overlap exists between this data set and proteomic studies of disparate viruses, including HIV-1 and influenza A, generated in multiple cell types. However, the great majority of proteins identified here have not been previously described to be incorporated within filovirus particles. Host proteins identified by liquid chromatography-linked tandem mass spectrometry could lack biological relevance because they represent protein contaminants in the virus preparation, or because they are incorporated within virions by chance. These issues were addressed using siRNA library-mediated gene knockdown (targeting each identified virion-associated host protein), followed by filovirus infection. Knockdown of several host proteins (e.g. HSPA5 and RPL18) significantly interfered with ebolavirus and marburgvirus infection, suggesting specific and relevant virion incorporation. Notably, select siRNAs inhibited ebolavirus, but enhanced marburgvirus infection, suggesting important differences between the two viruses. The proteomic analysis presented here contributes to a greater understanding of filovirus biology and potentially identifies host factors that can be targeted for antiviral drug development. |
| Used Model | 293T cells |
| DOI | 10.1074/mcp.M110.003418 |
