| Gene Name | EIF5A |
| HF Protein Name | Eukaryotic translation initiation factor 5A-1 |
| HF Function | Essential for virus replication |
| Uniprot ID | P63241 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 1984 |
| Host Factor (HF) Name in Paper | eIF5A |
| Gene synonyms | N.A. |
| Ensemble Gene ID | ENSG00000132507 |
| Ensemble Transcript | ENST00000336452 [P63241-2];ENST00000336458 [P63241-1];ENST00000416016 [P63241-1];ENST00000419711 [P63241-1];ENST00000571955 [P63241-1];ENST00000573542 [P63241-1];ENST00000576930 [P63241-1] |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0003723, GO:0003746, GO:0005634, GO:0005642, GO:0005643, GO:0005737, GO:0005789, GO:0005829, GO:0006406, GO:0006452, GO:0006611, GO:0006913, GO:0006915, GO:0008284, GO:0008612, GO:0016020, GO:0017070, GO:0043022, GO:0045901, GO:0045905, GO:0047485, GO:0070062, |
| MINT ID | P63241 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 600187 |
| PANTHER ID | PTHR11673 |
| PDB ID(s) | 1FH4, 3CPF, 5DLQ, |
| pfam ID | PF01287, |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Zaire ebolavirus |
| Virus Short Name | ZEBOV |
| Order | Bunyavirales |
| Virus Family | Filoviridae |
| Virus Subfamily | N.A. |
| Genus | Ebolavirus |
| Species | Zaire ebolavirus |
| Host | Bats, human and primates |
| Cell Tropism | N.A. |
| Associated Disease | Hemorragic fever |
| Mode of Transmission | Zoonosis, contact with body fluids |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=filo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/197/filoviridae |
| Virus Host DB link | N.A. |
| Paper Title | Polyamines and Hypusination Are Required for Ebolavirus Gene Expression and Replication |
| Author's Name | Michelle E. Olsen, Claire Marie Filone, Dan Rozelle, Chad E. Mire, Krystle N. Agans, Lisa Hensley, John H. Connora |
| Journal Name | mBio (American Society For Microbiology) |
| Pubmed ID | 27460797 |
| Abstract | Ebolavirus (EBOV) is an RNA virus that is known to cause severe hemorrhagic fever in humans and other primates : EBOV successfully enters and replicates in many cell types. This replication is dependent on the virus successfully coopting a number of cellular factors. Many of these factors are currently unidentified but represent potential targets for antiviral therapeutics. Here we show that cellular polyamines are critical for EBOV replication. We found that small-molecule inhibitors of polyamine synthesis block gene expression driven by the viral RNA-dependent RNA polymerase. Short hairpin RNA (shRNA) knockdown of the polyamine pathway enzyme spermidine synthase also resulted in reduced EBOV replication. These findings led us to further investigate spermidine, a polyamine that is essential for the hypusination of eukaryotic initiation factor 5A (eIF5A). Blocking the hypusination of eIF5A (and thereby inhibiting its function) inhibited both EBOV gene expression and viral replication. The mechanism appears to be due to the importance of hypusinated eIF5A for the accumulation of VP30, an essential component of the viral polymerase. The same reduction in hypusinated eIF5A did not alter the accumulation of other viral polymerase components. This action makes eIF5A function an important gate for proper EBOV polymerase assembly and function through the control of a single virus protein. |
| Used Model | BSR-T7/5 and A549 cells |
| DOI | 10.1128/mBio.00882-16 |
