| Gene Name | KPNA1 |
| HF Protein Name | Importin subunit alpha-5 |
| HF Function | Associated with virus infection, interact with VP24 of virus |
| Uniprot ID | P52294 |
| Protein Sequence | View Fasta Sequence |
| NCBI Gene ID | 3836 |
| Host Factor (HF) Name in Paper | KPNA1 |
| Gene synonyms | RCH2 |
| Ensemble Gene ID | ENSG00000114030 |
| Ensemble Transcript | ENST00000344337; |
| KEGG ID | Go to KEGG Database |
| Gene Ontology ID(s) | GO:0000018, GO:0005634, GO:0005643, GO:0005654, GO:0005737, GO:0005829, GO:0006309, GO:0006607, GO:0008139, GO:0008565, GO:0019054, GO:0030425, GO:0043657, GO:0061608, GO:0075733, |
| MINT ID | P52294 |
| STRING | Click to see interaction map |
| GWAS Analysis | Click to see gwas analysis |
| OMIM ID | 600686 |
| PANTHER ID | N.A. |
| PDB ID(s) | 2JDQ, 3TJ3, 4B18, , |
| pfam ID | PF00514, PF16186, PF01749, , |
| Drug Bank ID | N.A., |
| ChEMBL ID | N.A. |
| Organism | Homo sapiens (Human) |
| Virus Name | Zaire ebolavirus |
| Virus Short Name | ZEBOV |
| Order | Bunyavirales |
| Virus Family | Filoviridae |
| Virus Subfamily | N.A. |
| Genus | Ebolavirus |
| Species | Zaire ebolavirus |
| Host | Bats, human and primates |
| Cell Tropism | N.A. |
| Associated Disease | Hemorrhagic fever |
| Mode of Transmission | Zoonosis, contact with body fluids |
| VIPR DB link | http://www.viprbrc.org/brc/vipr_allSpecies_search.do?method=SubmitForm&decorator=filo |
| ICTV DB link | https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/197/filoviridae |
| Virus Host DB link | N.A. |
| Paper Title | Protein Interaction Mapping Identifies RBBP6 as a Negative Regulator of Ebola Virus Replication |
| Author's Name | Jyoti Batra, Judd F. Hultquist, Dandan Liu,6 Olena Shtanko, John Von Dollen, Laura Satkamp, Gwendolyn M. Jang, Priya Luthra, Toni M. Schwarz, Gabriel I. Small, Eusondia Arnett, Manu Anantpadma, Ann Reyes, Daisy W. Leung, Robyn Kaake, Paige Haas, Carson B. Schmidt, Larry S. Schlesinger, Douglas J. LaCount, Robert A. Davey, Gaya K. Amarasinghe, Christopher F. Basler, and Nevan J. Krogan |
| Journal Name | Cell |
| Pubmed ID | 30550789 |
| Abstract | Ebola virus (EBOV) infection often results in fatal illness in humans, yet little is known about how EBOV usurps host pathways during infection. To address this, we used affinity tag-purification mass spectrometry (AP-MS) to generate an EBOV-host protein-proteininteraction (PPI) map. We uncovered 194 high-confidence EBOV-human PPIs, including one between the viral transcription regulatorVP30 and the host ubiquitin ligase RBBP6. Domain mapping identified a 23 amino acid region within RBBP6 that binds to VP30. A crystal structure of the VP30-RBBP6 peptide complex revealed that RBBP6 mimics the viral nucleoprotein (NP) binding to the same interface of VP30. Knockdown of endogenous RBBP6 stimulated viral transcription and increased EBOV replication, whereas overexpression of either RBBP6 or the peptide strongly inhibited both. These results demonstrate the therapeutic potential of biologics that target this interface and identify additional PPIs that may be leveraged for novel therapeutic strategies. |
| Used Model | Hek293T and Huh7 |
| DOI | 10.1016/j.cell.2018.08.044 |
